EXPERTS IN TARGET VALIDATION
provitro
360° Services for IHC, ISH, TMAs and Pathology
Your Partner for Histology and Immunohistochemistry
Histological sections enable precise representation of tissue morphology, with staining outcomes affected by factors like buffer solution pH, staining duration, and fixation techniques.
Immunohistochemical staining, leveraging specific antibodies against tissue antigens, has greatly advanced in precision and breadth. Optimizing factors such as antibody concentration, incubation conditions, and temperature is crucial for high specificity and low background in staining, facilitating accurate tissue analysis.
IHC Services
How does it work?
Provitro’s competence is not limited to the development of protocols for both manual and automated IHC staining but includes, as well, target validation with antibodies on normal and tumour tissue samples which may be provided from our own tissue bank and ISH services.
False-negative results are ruled out by an in-process control system (iConTMA®).
Immunohistochemistry workflow
RNA in situ hybridisation
RNAscope® Technology is a novel in situ hybridisation (ISH) assay for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridisation. And, the double Z probe design with its relatively short target region allows for successful hybridisation of partially degraded RNA.
Tissue Microarrays (TMA) and iConTMA®
Peace of mind in screening and controls
Tissue microarrays are an ideal tool in high-throughput analysis for antigen distribution or validation of newly identified pathogenetic genes.
Tissue microarrays with up to 100 individual tissue samples on a single slide minimise variations in staining intensity, yielding consistent results.
Tissue spots with a diameter of up to 2 mm provide sufficient area for morphological evaluation. Tissue microarrays provide a rapid solution for localisation of DNA or protein molecules in normal and pathological human tissues.
Available TMAs:
- Normal TMA (off-target)
- Neoplastic (tumor) TMA
- Inflammatory + autoimmune TMA
- Cardiovascular TMA
- Customised TMA
Manufacturing Process
iConTMA®
iConTMA® are tissue slides for internal control of immunohistochemistry or in situ hybridisation. The slides contain specific positive and negative tissue spots providing enough space to add your tissue under investigation. The specificity of the reaction can be determined and the reactivity can be scored by comparing your sample with the iConTMA® spots.
